Chapter IV
نویسنده
چکیده
The addition of recombinant IL-12p75 (rIL-12p75) to naïve T cells promotes their differentiation toward a TH I-type cytokine patten by inducing IFN-y production. Therefore, it has been postulated that during the cognate interaction and formation of the immunological synapse between antigen-bearing DC and antigen-specific T cells, secretion of IFN-y and IL-12p75 would positively regulate each other. However, it is not clear during an immune response, which one of these cytokine is secreted first: IL-12p75 or IFNy? In this context, by using T cells from 5eC7 transgenic mice that are deficient in the IFN-y gene, this study poses the question: is secretion of IL-12p75 from DCs during cognate interaction is dependent on the presence of IFN-y secreted from T cells? Here we show that antigen-activated CD4+ but not naïve T cells from wild type (WT) or IFN-y-deficient mice induce IL-12p75 production during the cognate interaction with antigen-bearing DCs. Addition of recombinant IFN-y to the coculture of naïve T cells with DCs did not result in IL-12p75 production from DCs. Moreover, kinetic studies using antigen-activated T cells from the spleen of IFN-y-deficient and WT mice demonstrated that during the five days of coculture assay, secretion of IL-12p75 from DCs was independent of IFN-y, which was rapidly induced and declined over time. Furthermore, when WT or IFN-y-deficient mice were challenged with LPS or Superantigen [Staphylococcus enterotoxin A (SEA)], IL-12p75 could be detected in the serum of IFN-y-deficient mice injected with SEA but not
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